Determination by UV spectrophotometry of acetylcholine bromide content in test-strip for measuring whole blood cholinesterase activity

Objective  To establish an ultraviolet spectrophotometry method for the determination of acetylcholine bromide content in test strip for measuring whole blood cholinesterase activity, and validate the feasibility of this method.

Methods  The acetylcholine bromide in the test strip was eluted by using the difference in solubility between acetylcholine bromide and bromothymol blue(the two main components of the test strip). The standard curve of the method was established by using control solution containing 2.8, 5.6, 8.4, 11.2 and 14.0 mmol/L of acetylcholine bromide, and the standard curve linearity, accuracy, precision, durability and recovery of the method were validated.

Results  The standard curve of this method was linear and the average of linear correlation coefficients was 0.999 79. The accuracy, precision and durability of the method were good. The Intra- and inter-batch relative standard deviations were both less than 4%, and the recovery rates were between 94.90% and 106.22%.

Conclusion  This method could be used for quantitative detection of acetylcholine bromide content in test-strip for measuring whole blood cholinesterase activity.