Improvement of national standard method for determination of urinary manganese by GFAAS

Objective To improve national standard method of graphite furnace atomic absorption spectrometry for determination of manganese in urine.

Methods Palladium nitrate solution was used as chemical modifier and working curve method was performed with matrix matched calibration solutions, instead of standard curve method.

Results The linear relationship was obtained in the manganese concentration range of 0.0-12.0 μg/L. The regression equation was y = 0.007 50 x + 0.000 2 with correlation coefficient (R²) of 0.999 7. The LOD and LOQ were found to be 0.12 μg/L and 0.39 μg/L, respectively. Tests for recovery were made by standard addition method, and ranged from 98.6%-105.0% with RSD in the range of 0.77% - 2.15%.

Conclusion The results obtained suggest that the developed method has the advantages of low background absorption and good shape of peak. It demonstrates a good practical applicability for rapid, sensitive, precise and accurate determination of manganese in urine.